Muscle metabolism and nutrition

PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT

Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins (l), a number of modified analytical procedures ut.ilizing this reagent have been reported for the determination of proteins in serum (2-G), in antigen-antibody precipitates (7-9), and in insulin (10).

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding