@article{livak2001,
  title={Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method},
  author={Kenneth J. Livak and Thomas D. Schmittgen},
  year={2001},
  journal={Methods},
  doi={10.1006/meth.2001.1262},
  url={https://doi.org/10.1006/meth.2001.1262}
}